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goscripttm reverse transcription random hexamers master mix  (Promega)

 
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    Structured Review

    Promega goscripttm reverse transcription random hexamers master mix
    Goscripttm Reverse Transcription Random Hexamers Master Mix, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goscripttm+reverse+transcription+random+hexamers+master+mix/goscripttm+reverse+transcription+random+hexamers+master+mix/pm39136782-51-26-33
    Average 90 stars, based on 1 article reviews
    goscripttm reverse transcription random hexamers master mix - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: Novel CDKL5 targets identified in human iPSC-derived neurons.
    Article Snippet: .. Genes with a false discovery rate (FDR) of < 0.05 and a fold-change (FC) of > 2 were considered significant (Supplemental Table 1). cDNA was synthesized (GoScriptTM Reverse Transcription random hexamers master mix, Promega) and quantitative reverse-transcription PCR reactions were conducted with custom primers (Supplemental Table 2) and AccuPower® 2X GreenstarTM qPCR Master Mix (Bioneer Pacific) on a Roche LightCycler 480 II. .. Proteins were rapidly extracted from neurons in chilled RIPA buffer [25 mM Tris pH 8.0, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.5% sodium dodecyl sulfate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA)] containing protease and phosphatase inhibitors (protease and phosphatase inhibitor mini tablets, EDTA-free, Pierce), sonicated, protein concentration estimated (BCA kit, Pierce) then stored at −80 °C.

    Reverse Transcription:

    Article Title: Novel CDKL5 targets identified in human iPSC-derived neurons.
    Article Snippet: .. Genes with a false discovery rate (FDR) of < 0.05 and a fold-change (FC) of > 2 were considered significant (Supplemental Table 1). cDNA was synthesized (GoScriptTM Reverse Transcription random hexamers master mix, Promega) and quantitative reverse-transcription PCR reactions were conducted with custom primers (Supplemental Table 2) and AccuPower® 2X GreenstarTM qPCR Master Mix (Bioneer Pacific) on a Roche LightCycler 480 II. .. Proteins were rapidly extracted from neurons in chilled RIPA buffer [25 mM Tris pH 8.0, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.5% sodium dodecyl sulfate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA)] containing protease and phosphatase inhibitors (protease and phosphatase inhibitor mini tablets, EDTA-free, Pierce), sonicated, protein concentration estimated (BCA kit, Pierce) then stored at −80 °C.

    Polymerase Chain Reaction:

    Article Title: Novel CDKL5 targets identified in human iPSC-derived neurons.
    Article Snippet: .. Genes with a false discovery rate (FDR) of < 0.05 and a fold-change (FC) of > 2 were considered significant (Supplemental Table 1). cDNA was synthesized (GoScriptTM Reverse Transcription random hexamers master mix, Promega) and quantitative reverse-transcription PCR reactions were conducted with custom primers (Supplemental Table 2) and AccuPower® 2X GreenstarTM qPCR Master Mix (Bioneer Pacific) on a Roche LightCycler 480 II. .. Proteins were rapidly extracted from neurons in chilled RIPA buffer [25 mM Tris pH 8.0, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.5% sodium dodecyl sulfate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA)] containing protease and phosphatase inhibitors (protease and phosphatase inhibitor mini tablets, EDTA-free, Pierce), sonicated, protein concentration estimated (BCA kit, Pierce) then stored at −80 °C.

    Real-time Polymerase Chain Reaction:

    Article Title: Novel CDKL5 targets identified in human iPSC-derived neurons.
    Article Snippet: .. Genes with a false discovery rate (FDR) of < 0.05 and a fold-change (FC) of > 2 were considered significant (Supplemental Table 1). cDNA was synthesized (GoScriptTM Reverse Transcription random hexamers master mix, Promega) and quantitative reverse-transcription PCR reactions were conducted with custom primers (Supplemental Table 2) and AccuPower® 2X GreenstarTM qPCR Master Mix (Bioneer Pacific) on a Roche LightCycler 480 II. .. Proteins were rapidly extracted from neurons in chilled RIPA buffer [25 mM Tris pH 8.0, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.5% sodium dodecyl sulfate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA)] containing protease and phosphatase inhibitors (protease and phosphatase inhibitor mini tablets, EDTA-free, Pierce), sonicated, protein concentration estimated (BCA kit, Pierce) then stored at −80 °C.



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